Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1983-01-18
1985-12-17
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435253, 435317, 935 11, 935 29, 935 75, 536 27, C12P 2100, C12N 1500, C12N 120, C12N 100
Patent
active
045593000
ABSTRACT:
A method for expressing a functional polypeptide in Streptomyces comprises transforming a Streptomyces host cell with a recombinant DNA expression vector and then culturing the transformed cell under conditions suitable for cell growth. The recombinant DNA expression vector comprises the veg or any other homologous Bacillus promoter, a naturally occurring or modified ribosome binding site-containing DNA sequence and a gene that codes for a functional polypeptide such as human pre-proinsulin. The method is specifically exemplified by use of expression plasmids pOW529, pOW539 and transformants, Streptomyces ambofaciens/pOW529 and Streptomyces ambofaciens/pOW539. The method is broadly applicable and is particularly useful in economically important Streptomyces taxa.
REFERENCES:
patent: 344710 (1886-06-01), Bennett
patent: 4273875 (1981-06-01), Manis
patent: 4360597 (1982-11-01), Bibb et al.
patent: 4416994 (1983-11-01), Nakatsukasa et al.
patent: 4436815 (1984-03-01), Hershberger et al.
patent: 4460688 (1984-07-01), Sanders et al.
Moran, Jr., et al., Molecular and General Genetics, 1982, vol. 186, pp. 339-346.
Palva, I., 1982, Gene 19:81.
Palva, I. et al., 1982, Proc. Nat'l. Acad. Sci., USA, 79:5582.
Derwent Abstract, 37323, E/19-Abstract of Belgium No. BE-891-659.
Bibb and Cohen, 1982, Mol. Gen. Genet., 187:265-277.
Westpheling, et al., 1985, Nature, vol. 313.
Fayerman Jeffrey T.
Kovacevic Steven
Miller James R.
Richardson Mark A.
Dahling Gerald V.
Eli Lilly and Company
Huleatt Jayme A.
Tanenholtz Alvin E.
Whale Arthur R.
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