Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1983-02-28
1985-11-26
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 68, 435 70, 4351723, 435317, 536 27, 935 16, 935 17, 935 18, C12P 2100, C12P 2102, C12N 1500, C12N 100, C07H 2104
Patent
active
045554862
ABSTRACT:
A method is described for constructing double-stranded DNA using messenger RNA templates. DNA strands complementary to the templates are formed using a non-specific 3' hydroxy terminus primer. After separation, the DNA single strands are disabled from self priming by blocking the free 3' hydroxy. Primer segments are then provided complementary to a portion of the amino terminus-coding end of the desired single strands. These primer segments are then extended with a suitable polymerase to complete the complementary strands.
REFERENCES:
patent: 4401759 (1983-08-01), Rubin et al.
Houghton and Stewart et al.; Nucl. Acids Res. 8, 1913 (1980).
Houghton, Eaton et al.; Nucl. Acids Res. 8, 2885 (1980).
Wickens, M. P. et al., J. Biol. Chem., 253:2483-2495 (1978).
Buell, G. N. et al., J. Biol. Chem. , 253:2471-2482 (1978).
Goodman, H. M. and MacDonald, R. J. Methods in Enzmology, 68:75-91 (1979).
Efstratiadis et al.; Cell 7, 279 (1976).
Chan et al.; Proc. Natl. Acad. Sci., USA 76, 5036 (1979).
Sanger et al., "DNA sequencing with chain-terminating inhibitors", Proc. Natl. Acad. Sci. USA 74: 5463 (1977).
Houghton et al., "The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers", Nucleic Acids Res. 8: 1913 (1980).
Houghton et al., "The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcripase", Nucleic Acids Res. 8: 2885.
Efstratiadis et al., "Enzymatic in vitro synthesis of globin genes", Cell 7: 279 (1976).
Chan et al., "Construction and selection of recombinant plasmids containing full-length complementary DNAs corresponding to rat insulins I and II", Proc. Natl. Acad. Sci. USA 76: 5036 (1979).
Bahl Chander P.
Nunberg Jack
Cetus Corporation
Fineman Elliott L.
Halluin Albert P.
Hasak Janet E.
Martinell James
LandOfFree
Method for using an amino-terminus DNA sequence to synthesize a does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for using an amino-terminus DNA sequence to synthesize a , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for using an amino-terminus DNA sequence to synthesize a will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-269833