Method for using a novel transcriptional- and translational-acti

Details Method for using a novel transcriptional- and translational-acti Method for using a novel transcriptional- and translational-acti

1984-09-13

1988-06-21

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435169, 435170, 4351721, 4351723, 435183, 435243, 435253, 4353171, 435820, 435822, 435886, 536 27, 935 23, 935 27, 935 41, 935 56, 935 75, C12N 1500, C12N 500

Patent

active

047525770

ABSTRACT:
The present invention is a method for expressing functional polypeptides in Streptomyces using a recombinant DNA expression vector comprising a novel transcriptional- and translational- activating sequence. The novel activating sequence can be synthesized by conventional methods and used in Streptomyces expression vectors. One such vector, plasmid pFJ350, expresses and confers hygromycin resistance in Streptomyces host cells.

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