Method for two-dimensional conformation-dependent separation...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Reexamination Certificate

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10505585

ABSTRACT:
Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that linear nucleic acid fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different, such that in one of said dimensions electrophoresis allows separation of linear nucleic acid fragments based on conformation and length, and in the other of said dimensions electrophoresis allows separation of the sample fragments based substantially on length. Said difference is preferably established with a chemical agent capable of reducing conformational differences between linear nucleic acids fragments.

REFERENCES:
patent: 5874212 (1999-02-01), Prockop et al.
patent: WO-92-13101 (1992-08-01), None
patent: WO-95/11993 (1995-05-01), None
patent: WO-97/39149 (1997-10-01), None
Uitterlinden et al, Proc. Natl. Acad. Sci. USA. 86: 2742 (1989).
Woolley et al, Cancer chemother. pharmacol. 44(6), 511 (1999), abstract only.
Hansen et al, Nucleic Acids Res. 24(5), 859 (1996), abstract only.
Tang et al, Bulletin Chem. Soc. Japan 71(7), 1725 (1998), abstract only.
Gao et al, Biochemistry 32: 9639 (1993), abstract only.
Kovar, H. et al., “Two Dimensional Single-Strand Conformation Polymorphism Analysis: A Useful Tool for the Detection of Mutations in Long DNA Fragments”, Nucleic Acids Research. England. vol. 19, No. 13.
Thomar et al., “From Genomic to Representational Mismatch Scanning”, American Journal of Human Genetics, vol. 71, No. 4. p. 398. Oct. 2002.
Gunnarsson, G.H. et al., “2-D Conformation-Dependent Electrophoresis for Analysis of Complex DNA Samples (2D-CDE).” ###American Journal of Human Genetics, vol. 71, No. 4, p. 400. Oct. 2002.

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