Drug – bio-affecting and body treating compositions – Lymphokine
Patent
1986-12-31
1989-01-31
Kight, John
Drug, bio-affecting and body treating compositions
Lymphokine
424 88, 436518, 436523, 436527, 210656, 210927, 530413, G01N 33552, G01N 33543, B01D 1508
Patent
active
048014494
ABSTRACT:
An immunoadsorbent material for removing IgG and IgG-complexes from biological fluids is prepared by covalently binding protein A to a solid-phase silica matrix. It has been found that particularly stable, high-capacity immunoadsorbents are obtained by derivatizing the silica with amino and/or carboxyl groups, and reacting the protein A with a carbodiimide at a pH in the range from 3.5 to 4.5. Binding through free hydroxyl groups may be achieved with cyanogen halides at a pH in the range from 11.0 to 11.5. After acid washing (pH 2.0-2.5) to remove non-covalently bound protein A, the immunoadsorbent may be employed in a column for therapeutic treatment of various cancers and autoimmune disorders where IgG-complexes are implicated as suppressing factors in inhibiting a normal immune response. The column has been successfully employed in treating patients suffering from Kaposi's sarcoma.
REFERENCES:
Terman et al., "Preliminary Observations of the Effects on Breast Adenocarcinoma of Plasma Perfused Over Immobilized Protein A," New Eng. Journ of Med, 305:1195-1200, Nov/1981.
Holohan et al., "Regression of Canine Mammary Carcinoma After Immunoadsorption Therapy" Cancer Research 42:3663-3668, Sep./1982.
Kiprov et al., "Extracorporeal Perfusion of Plasma over Immobilized Protein A in a Patient with Kaposi's Sarcoma and Acquired Immunodeficiency", Journ. of Biol. Res, Mod., 3:341-346, 1984.
Bansal et al., "Ex Vivo Removal of Serum IgG in a Patient with Colon Carcinoma", Cancer, vol. 42, pp. 1-18, Jul./1978.
Messerschmidt et al., "Phase I Trial of Staphylococcus Aureus Cowan I Immunoperfusion" Cancer Treatment Reports, vol. 66, No. 12, pp. 2027-2031, Dec./1982.
Besa et al., "Specific Immunoadsorption of IgG Antibody in a Patient with Chronic Lymphocytic Leukemia and Autoimmune Hemolytiz Anemia" Am. Journ. of Med, vol. 71, pp. 1035-1040, Dec./1981.
Balint, Jr. Joseph P.
Jones Frank R.
IMRE Corporation
Kight John
Nutter Nathan M.
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