Method for trapping nucleic acids using divalent metal ions

Details Method for trapping nucleic acids using divalent metal ions Method for trapping nucleic acids using divalent metal ions

2005-08-18

2009-11-10

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S007100, C435S007200, C422S068100, C536S023100, C536S024300

Reexamination Certificate

active

07615347

ABSTRACT:
A method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and having a pH of 12 or higher with the surface of the solid phase substrate on which divalent metal ions are immobilized, and a method for trapping nucleic acids on a surface of a solid phase substrate, which comprises the step of contacting a sample containing nucleic acids and a magnesium compound, of which pH is adjusted to be 12 or higher, with the surface of the solid phase substrate.

REFERENCES:
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patent: 6689478 (2004-02-01), Laguitton
patent: 2005/0037351 (2005-02-01), Kanno et al.
patent: 0 580 305 (1994-01-01), None
patent: 6-078769 (1994-03-01), None
patent: WO-03/006650 (2003-01-01), None
Murphy et al., Biotechnol. Prog. 2003, vol. 19, No. 3, pp. 982-986.

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