Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target
Patent
1996-03-01
1997-12-02
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Cell membrane or cell surface is target
4353201, C12N 1300, C12N 1563
Patent
active
056935129
ABSTRACT:
The present invention provides an efficient, cost effective method for transforming, including stably transforming, plants including monocots, dicots and gymnosperms. The method of the present invention, which is less labor intensive than typical conventional transformation methods, comprises combining the plant and a vector which contains the foreign nucleic acid to be introduced into the plant sample. Preferably the plant sample is sonicated in the presence of the vector. The vector is preferably non-tumor inducing bacteria, more preferably Agrobacterium. The sonication duration is less than 15 minutes, preferably less than 6 minutes more preferably 60 seconds or less. After sonicating the plant sample is cultured under conditions that induce morphogenesis to produce plant tissue that will mature into a transformed plant.
REFERENCES:
patent: 5416011 (1995-05-01), Hinchee et al.
patent: 5480789 (1996-01-01), Firoozabady et al.
Joersbo et al. Direct gene transfer to plant protoplast by mild sonication. Plant Cell Reports. 9:207-210, 1990.
Zhang et al. Efficient transformation of tobacco by ultrasonication. Bio Technology. 9:996-997, 1991.
Joersbo et al. Sonication: a new method for gene transfer to plants. Physiologia Plantarum. 85:230-234, 1992.
Van Vloten-Doting, et al. Plant-virus-based vectors for gene transfer will be of limited use because of the high error frequency during viral RNA synthesis. Plant Molecular Biology. 4:323-326, 1985.
De Greve et al. Regeneration of normal and fertile plants that express octopine synthase, from tobacco crown galls after deletion of tumour-controlling functions. Nature. 300:752-755, 1982.
"Agrobacterium-mediated Transformation of Walnut Somatic Embryos and Regeneration of Transgenic Plants" by McGranahan, et al. Biotechnology, vol. 6, Jul. 1988, pp. 800-804.
"Direct gene transfer to plant protoplasts by mild sonication" by Joersbo, et al., Plant Cell Reports, (1990)9: 207-210.
"Sonication: A new method for gene transfer to plants" by Joersbo, et al., Physiologia Plantarium, 85: 230-234, Copenhagen, 1992.
"Gene Transfer into Maize by Ultrasonication" by Hong, et al., Agricultural Biotechnology: Proceedings of Asia-Pacific Conf. on Agricultural Biotechnology, Aug. 1992, pp. 311-312.
"Efficient Transformation of Tobacco by Ultrasonication" by Zhang, et al., Biotechnology, 9: 996-997 (1991).
"Direct Gene Transfer into Wheat Somatic Cells by Ultrasonic Treatment of Immature Embryos" by Xu, et al., Agricultural Biotechnology: Proceedings of Asia-Pacific Conf. on Agricultural Biotechnology, Aug. 1992, pp. 294-295.
"Agrobacterium-mediated delivery of infectious maize streak virus into maize plants" by Grimsley, et al., Nature, vol. 325, Jan. 1987, pp. 177-179.
"Regeneration of Intact Tobacco Plants Containing Full Length Copies of Genetically Engineered T-DNA and Transmission of T-DNA to R1 Progeny" by Barton, et al., Cell, vol. 32, pp. 1033-1043, Apr. 1983.
Finer John J.
Trick Harold N.
Robinson Douglas W.
The Ohio State Research Foundation
Wai Thanda
LandOfFree
Method for transforming plant tissue by sonication does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for transforming plant tissue by sonication, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for transforming plant tissue by sonication will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-800516