Method for transforming monocotyledons

Details Method for transforming monocotyledons Method for transforming monocotyledons

1994-05-03

1997-01-07

Benzion, Gary

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351721, 4352404, 43524045, 43524049, 4352405, 43524054, 4353201, 4352522, 800205, 935 52, 935 55, 935 67, C12M 1500, C12R 141

Patent

active

055916163

DESCRIPTION:

BRIEF SUMMARY
TECHNICAL FIELD

The present invention relates to a method for transforming monocotyledons.


BACKGROUND ART

Conventional methods for transforming monocotyledons include the electroporation method, the polyethylene glycol method (PEG method), the particle gun method and so on.
The electroporation method is a method in which protoplasts and the desired DNA are mixed, and holes are formed in the cell membranes by electric pulse so as to introduce the DNA into the cells, thereby transforming the cells. This method currently has the highest reproducibility of the conventional methods and various genes have been introduced into monocotyledons, especially into rice plants by this method (Toriyama K. et al., 1988; Bio/Technol. 6:1072-1074, Shimamoto K. et al., 1989; Nature 338:274-276, Rhodes C. A. et al., 1989; Science 240:204-207). However, this method has the problems that 1) it can be applied only to the plant species for which the system for regenerating plants from protoplasts has been established, 2) since it takes several months to regenerate plants from the protoplasts, a long time is required to obtain transformants, and that 3) since the culture period is long, the frequency of emergence of mutants during the culture is high accordingly, so that the probability of obtaining normal transformants is decreased.
The PEG method is a method in which the desired gene and protoplasts are mixed and the mixture is treated with PEG, thereby introducing the gene into the protoplasts. This method is different from the electroporation method in that PEG is used instead of the electric pulse. The efficiency of introducing the gene is thought to be somewhat lower than the electroporation method. Although there is a report that transformants were obtained by this method, this method is not widely used. Since protoplasts are used, this method has the same problems as in the electroporation method (Zhang W. et al., 1988; Theor. Appl. Genet. 76:835-840, Datta S. K. et al., 1990; Bio/Technol. 8:736-740).
The particle gun method is a method in which the desired gene is attached to fine metal particles, and the metal particles are shot into cells or tissues at a high speed, thereby carrying out the transformation. Thus, according to this principle, transformation may be performed on any tissues. Therefore, this method is effective for transforming the plant species for which the systems for regenerating plants from protoplasts have not been established. The efficiency of transformation varies depending on the selection after the gene was shot. There is no data which compare the efficiency of this method with that of the electroporation method (Gordon-Kamm W. J. et al., 1990; Plant Cell 2:603-618, Fromm M. E. et al., 1990; Bio/Technol. 8:833-839, Christou P. et al., 1991; Bio/Technol. 9:957-962).
Other methods include 1) culturing seeds or embryos with DNA (Topfer R. et al., 1989; Plant Cell 1:133-139, Ledoux L. et al., 1974 Nature 249:17-21); 2) treatment of pollen tube (Luo and Wu 1988; Plant Mol. Biol. Rep. 6:165-), 3) liposome method (Caboche M. 1990; Physiol. Plant. 79:173-176, Gad A. E. et al., 1990:177-183) and 4) microinjection method (Neuhaus G. et al., 1987; Theor. Appl. Genet. 75:30-36). However, these methods have problems in the efficiency of transformation, reproducibility or applicability, so that these methods are not commonly used.
On the other hand, a method for introducing a gene using the Ti plasmid of bacteria belonging to the genus Agrobacterium as a vector is widely used for transforming dicotyledons such as tobacco, petunia, rape and the like. However, it is said that the hosts of the bacteria belonging to genus Agrobacterium are restricted to dicotyledons and that monocotyledons are not parasitized by Agrobacterium (De Cleene M. 1976; Bot. Rev. 42:389-466).
As for transformation of monocotyledons by Agrobacterium, although transformation of asparagus (Bytebier B. et al., 1987: Proc. Natl. Acad. Sci. USA, 84:5345-5349) and of Dioscorea bulbifera (Schafew et al., 1987; Nature 327:529-532) has been repo

REFERENCES:
patent: 4940838 (1990-07-01), Schilperoort et al.
patent: 5149645 (1992-09-01), Hoekema et al.
patent: 5164310 (1992-11-01), Smith et al.
patent: 5177010 (1993-01-01), Goldman et al.
patent: 5187073 (1993-02-01), Goldman et al.
Mooney et al. (1991) Plant Cell Tissue & Organ Culture, vol. 25, pp. 209-218.
Sangwan et al. (1991) Mol. and Gen. Genetics, vol. 230, pp. 475-485.
Chem. Abstracts (1988) vol. 109(13), Abstract No. 105884.
Yao et al. (1990) Chinese J. Bot 2(2):81-87.
Chan et al. (1993) Plant Molecular Biology 22:491-506.
Lee et al. (1991) Proc. Nat'l. Acad. Sci. USA 88(15)6389-93.
Li et al. (1991) Science in China (Series B), vol. 34(8), pp. 923-931.
Chan et al. (1992) Plant Cell Physiol. 33(5) pp. 577-583.
Li et al. (1991) Science in China (Series B), vol. 34(1), pp. 54-63.
Lupotto et al. (1988) Maydica 33:163-177.
Mikami et al. (1988) Plant Cell, Tissue and Organ Culture 12:311-314.
Saito et al. (1992) Theoretical and Applied Genetics 83:679-683.
Komari et al. (1989) Theoretical and Applied Genetics 77:547-552.
Komari (1989) Plant Science (1989) 60:223-229.
Drlica et al. (1974) Proc. Natl. Acad. Sci. USA 71:3677-3681.
Pietrzak et al. (1986) Nucleic Acids Research 14:5857-5868.
Ohta et al. (1990) Plant Cell Physiol. 31(6):805-813.
Murashige et al. (1962) Physiologia Plantarum 15:473-497.
Toriyama et al. (1985) Plant Science 41:179-183.
Ditta et al. (1980) Proc. Natl. Acad. Sci. USA 77:7347-7351.
Horsch et al. (1984) Science 223:496-499.
Herrera-Estrella et al. (1983) The EMBO Journal 2(6):987-995.
Jin et al. (1987) Journal of Bacteriology 169(10):4417-4425.
Komari et al. (1986) Journal of Bacteriology 166(1):88-94.
Linsmaier et al. (1965) Physiologia Plantarium 18:100-127.
Hood et al. (1984) Biotechnology 2:702-709.
Hood et al. (1986) Journal of Bacteriology 168(3):1283-1290.
Toriyama et al. (1988) Bio/Technology 6:1072-1074.
Shimamoto et al. (1989) Nature 338:274-277.
Rhodes et al. (1988) Science 240:204-207.
Zhang et al. (1988) Theoretical and Applied Genetics 76:835-840.
Datta et al. (1990) Bio/Technology 8:736-740.
Gordon-Kamm et al. (1990) The Plant Cell 2:603-618.
Christou et al. (1991) Bio/Technology 9:957-962.
Fromm et al. (1990) Bio/Technology 8:833-839.
T opfer et al. (1989) The Plant Cell 1:133-139.
Caboche et al. (1990) Physiol. Plant. 79:173-176.
Gad et al. (1990) Physiologia Plantarum 79:177-183.
Neuhaus et al. (1987) Theoretical and Applied Genetics 75:30-36.
The Botanical Review 390-466.
Bytebier et al. (1987) Proc. Natl. Acad. Sci. USA 84:5345-5549.
Sch afer et al. (1987) Nature 327:529-533.
Grimsley et al. (1987) Nature 325(8):177-179.
Grimsley et al. (1988) Bio/Technology 6:185-189.
Grimsley et al. (1989) Mol Gen Genet 217:309-316.
Gould et al. (1991) Plant Physiol. 95:426-434.
Chih-ching (1981) Plant Tissue Culture, Pitman, Boston, pp. 43-50.
Gritz et al. (1983) Gene 25:179-188.
De Greve et al. (1981) Plasmid 6:235-248.
Luo et al. (1988) Plant Molecular Biology Reporter 6(3):165-174.
Raineri et al. Bio/technology vol. 8 pp. 33-38 Jan. (1990).
Potrykus. Bio/technology Jun. (1990) pp. 535-542.

Affiliated with

Also associated with

Rating

Method for transforming monocotyledons does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for transforming monocotyledons, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for transforming monocotyledons will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1763455