Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1998-06-12
1999-12-07
Fox, David T.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
4354301, 435431, 435469, 435470, 800293, 800294, 800298, A01H 400, A01H 100, C12N 1582, C12N 1584
Patent
active
059982071
ABSTRACT:
Protocols for ogranogenic regeneration of cotton and kenaf are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO.sub.3. These explants, prior to shoot initiation, may be transformed with exogenous DNA either through inoculation with a Agrobacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface.
REFERENCES:
Banks et al. Agrobacterium--mediated transformation of Kenaf (Hibiscus cannabinus L.) with the B-Glucuronidase (GUS) Gene. Plant Molecular Biology Reporter, vol. 11(2) : 101-104, 1993.
MCLean et al. Callus induction and adventitious organogenesis of Kenaf (Hibiscus cannabinus L), Plant Cell Reports 11 : 532-534, 1992.
Lim Teong-Kwee
Reichert Nancy A.
Young Margaret M.
Fox David T.
Grunberg Anne Marie
Kelber Steven B.
Mississippi State University
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