Chemistry: molecular biology and microbiology – Vector – per se
Patent
1983-09-26
1987-09-01
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Vector, per se
435 68, 4351723, 435842, 935 29, 935 56, C12N 1500, C12N 100, C12P 2100, C12R 1145
Patent
active
046908978
ABSTRACT:
Method for transforming anaerobic microorganisms. Anaerobic microorganisms are induced to form L-forms. Genetic material capable of inducing the desired phenotype is introduced into the L-forms, after which the L-forms may be caused to regenerate their cell wall. These methods are also useful for obtaining desired biological products. Additionally, shuttle vectors capable of transforming both aerobic and anaerobic microorganisms are set forth.
REFERENCES:
patent: 4332900 (1982-06-01), Manis et al.
patent: 4477576 (1984-10-01), Chang et al.
patent: 4493893 (1985-01-01), Mielenz et al.
Allcock et al, App and Env Micro, vol. 43(3) pp. 719-721, Mar. 1982, "Clostricium Acetobutylicum Protoplast Formation and Regeneration".
Rood et al, Plasmid, vol. 1, pp. 563-570, 1978, "Identification of a Transferable Tretracycline Resistance Plasmid CW3 from Clostridium Perfringens".
Madoff, Subrelle The Prokcyotes, Springer-Verlag, vol. VII pp. 2225-2236, 1981.
Evans Ronald J.
Heefner Donald L.
Kopp Beatrice J.
Squires Charles H.
Yarus Michael J.
Synergen Associates, Inc.
Teskin Robin Lyn
Wiseman Thomas G.
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