Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2004-09-29
2008-10-14
Ketter, James S (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S458000
Reexamination Certificate
active
07435595
ABSTRACT:
The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.
REFERENCES:
patent: 4179337 (1979-12-01), Davis et al.
patent: 5888732 (1999-03-01), Hartley et al.
patent: 5965404 (1999-10-01), Buschle et al.
patent: 0 908 521 (1999-04-01), None
Bian, J. et al. “Nuclear Translocation of HIV-1 Matrix Protein P17: The Use ofAequorea victoriaGreen Fluorescence Protein in Protein Tagging and Tracing,”Protein Studies Program, Oklahoma Medical Reserch Foundation, Univ. of Okla. Health Sci. Ctr.Oklahoma City, OK 73104 1995, 1 page total (abstract).
Boulikas, T. and Martin, F. “Histones, Protamine, and Polylysine but not Poly(E:K) Enhance Transfection Efficiency,”International J. of Oncology1997, pp. 317-322, vol. 10.
Branden, L.J. et al. “A Peptide Nucleic Acid-nuclear Localization Signal Fusion that Mediates Nuclear Transport of DNA,”Nature BiotechnologyAug. 1999, pp. 784-787, vol. 17.
Elliott, G and O'Hare, P. “Intercellular Trafficking and Protein Delivery by a Herpesvirus Structural Protein,”CellJan. 24, 1997, pp. 223-233, vol. 88.
Esser, D. et al. “The HU Protein from Thermotoga maritima: Recombinant Expression, Purification and Physicochemical Characterization of an Extremely Hyperthermophilic DNA-binding Protein,”J. Mol. Biol.1999, pp. 1135-1146, vol. 291.
Esser, D. et al. “A Hyperthermostable Bacterial Histone-like Protein as an Efficient Mediator for Transfection of Eukaryotic Cells,”Nature BiotechnologyNov. 2000, pp. 1211-1213, vol. 18.
Fritz, D. et al. “Gene Transfer into Mammalian Cells Using Histone-Condensed Plasmid DNA,”Human Gene TherapyAug. 1996, pp. 1395-1404, vol. 7.
Hentschel, C.C. and Birnsteil, M.L. “The Organization and Expression of Histone Gene Families,”Cell1981, pp. 301-313, vol. 25, No. 2.
Ziemienowicz, A. et al. “Import of DNA into Mammalian Nuclei by Proteins Originiating from a Plant Pathogenic Bacterium,”Proc. Natl. Acad. Sci USAMar. 1999, pp. 3729-3733, vol. 96.
Ledley, F.D.; “Nonviral gene therapy: the promise of genes as pharmaceutical products”; 1995,Hum. Gene Ther.(Abstract), vol. 6, No. 9, pp. 1129-1144.
Li, J.Y. et al.; “Histones and histone-like DNA-binding proteins: correlations between structural differences, properties and functions”; 1999,Microbiology, vol. 145, pp. 1-3.
Boehm Gerald
Esser Dirk
ACGT ProGenomics AG
Ketter James S
Townsend and Townsend / and Crew LLP
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