Method for transfected cell selection

Details Method for transfected cell selection Method for transfected cell selection

1993-09-08

1995-05-02

Beisner, William H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 34, 435240241, C12Q 124, C12Q 104, C12N 500

Patent

active

054118727

ABSTRACT:
A method for selecting transfected cell clones employs a transparent container that includes a lower-most surface that is covered with a transparent, thin sheet. The method includes the steps of: growing transfected cell clones on the transparent sheet within a growth medium in the transparent container; excising areas of the transparent sheet that support a transfected cell clone; and placing the excised transfected cell clone in a cell growth medium for further replication. The transparent sheet contained within the transparent container is provided with a peripheral adhesive area that enables it to remain in place when a transfected cell clone is excised.

REFERENCES:
patent: 4237223 (1980-12-01), Metz
patent: 4304866 (1981-12-01), Green
patent: 4684613 (1987-08-01), Barrere
patent: 5137812 (1992-08-01), Matner
Klebe R., Cultivation of Mammalian Cells in Heat-Sealable . . . Exp Cell Research '88 pp. 316-319 1990.
Costar Catalog 1992, Cambridge Mass. (800) 492-1110.

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