Chemistry: physical processes – Physical processes – Crystallization
Patent
1976-10-04
1978-01-03
Marantz, Sidney
Chemistry: physical processes
Physical processes
Crystallization
260112R, 260113, G01N 3316, G01N 2100, G01N 2152, G01N 3108
Patent
active
040664058
ABSTRACT:
A method for the total protein fractionation of saliva and for isolating a novel zinc protein found to be a component thereof is disclosed. Saliva was fractionated using three protein parameters and zinc concentration by a molecular sieving process. The zinc protein was isolated and purified from the saliva of subjects with normal taste acuity by gel filtration and ion exchange chromatography maximizing these parameters. The zinc protein has a molecular weight of 37,000 and does not appear to have subunits. It is composed of 8% histidine residues and has two moles of zinc per mole of protein. A decreased concentration of the protein in saliva has been associated with abnormal taste acuity.
REFERENCES:
patent: 3763136 (1973-10-01), Huber
"Advances in Protein Chem.," C. B. Anfinsen et al., eds., Academic Press, vol. 17, 209-226, 303-315, 378-390 (1962).
Croft, Biochem. J., vol. 130, 303-305 (1972).
Chemical Abstracts I, 77: 84718r (1972).
Chemical Abstracts II, 79: 50026q (1973).
Chemical Abstracts III, 72: 74756j, (1970).
Chemical Abstracts IV, 83: 93311d (1975).
Marantz Sidney
The United States of America as represented by the Department of
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