Radiant energy – Ionic separation or analysis – Ion beam pulsing means with detector synchronizing means
Patent
1996-11-27
1998-03-31
Berman, Jack I.
Radiant energy
Ionic separation or analysis
Ion beam pulsing means with detector synchronizing means
250282, H01J 4940
Patent
active
057341613
ABSTRACT:
The invention relates to methods for the structural analysis of large substance molecules, preferably of chain molecules such as peptides for example, by scanning daughter or fragment ion mass spectra in time-of-flight mass spectrometers with reflectors. The invention consists in the differentiation between daughter ions from spontaneous fragmentations of ions by high-energy collisions and those from delayed fragmentations of metastable ions, by separately detecting the different kinds of ions using a special electrostatic energy filtration by a short Einzel lens directly behind the collision zone. Spontaneously decomposing ions leave the fragmentation zone with smaller kinetic energy due to the loss of mass. Daughter ions from spontaneous decompositions on the one hand, and from metastable decompositions on the other, display characteristic differences which can be used for the determination of structure. Spontaneously decomposing peptide ions preferably show, for example, simultaneously occurring fragmentations of the main and side chains on the then terminal links, while metastable decompositions display no fragmentations of the side chains. In this way, for example, it can be distinguished whether the terminal amino acid is leucine or isoleucine, even though both amino acids have the same mass.
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Berman Jack I.
Bruker-Franzen Analytik GmbH
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