Method for the volumetric differentiation of blood cells types

Details Method for the volumetric differentiation of blood cells types Method for the volumetric differentiation of blood cells types

1986-10-02

1988-05-17

Richman, Barry S.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 17, 436 18, G01N 3350

Patent

active

047450711

ABSTRACT:
Improved reagents and methods for obtaining distinct volumetric differentiation of platelets, erythocytes and certain leukocyute subpopulations are disclosed. 1,3-dimethylurea is disclosed as a cell stabilizing agent for use in the blood diluent reagent. A hematology analyzer detergent is described wherein the addition of a wetting agent to the diluent of the instant invention provides the necessary attributes of an automatic analyzer detergent. The lysing reagent exploits the selective and intrinsically gentle lytic qualities of the dodecyl quaternary ammonium salt. The improved method modifies the leukocytes so that the frequent lymphocyte and neutrophil subpopulations are well separated on a volume scale, permitting quantitative evaluation of infrequent and rare leukocyte subpopulations as well as improved enumeration of the lymphocytes and neutrophils.

REFERENCES:
patent: 2745826 (1956-05-01), Semenoff et al.
patent: 3236733 (1966-02-01), Karsten et al.
patent: 3281366 (1966-10-01), Judge et al.
patent: 3874852 (1975-04-01), Hamill
patent: 4099917 (1978-07-01), Kim
patent: 4185964 (1980-01-01), Lancaster
patent: 4213876 (1980-07-01), Crews et al.
patent: 4271123 (1981-06-01), Curry et al.
patent: 4286963 (1981-09-01), Ledis et al.
patent: 4346018 (1982-08-01), Carter et al.
patent: 4485175 (1984-11-01), Ledis et al.
patent: 4493821 (1985-01-01), Harrison
patent: 4521518 (1985-06-01), Carter et al.
patent: 4528158 (1985-07-01), Gilles et al.
patent: 4529705 (1985-07-01), Larsen
patent: 4617275 (1986-10-01), Matsuda et al.
Bessman, Tex. Med., vol. 80, pp. 33-35, 1984.
Allen et al., Am. J. Clin. Path., vol. 33, pp. 553-556, 1960.
Van Dilla et al., I.V.P.S.E.B.M., vol. 125, pp. 367-370, 1967.
Hatch et al., Am. J. Clin. Path., vol. 36, pp. 220-223, 1960.
Davis et al., J. Med. Lab. Technol., vol. 26, pp. 26-30, 1969.
Cox et al., Am. J. Clin. Path., vol. 84, No. 3, pp. 297-305, 9/85.
D'Angelo et al., Am. J. Clin. Path., vol. 34, pp. 220-223, 1961.
Adams et al., Phip. Med. Biol., vol. 12, pp. 79-92, 1967.
Torlontano et al., Acta Haemat., vol. 45, pp. 325-329, 1971.
Saslow, "The Volume of Rabbit Erythrocytes in Solutions of Various Tonicities", PhD. Thesis, New York University, 1931.
Wintrobe, ed., Clinical Hematology, 6th edition, Lea & Febiger, Philadelphia, 1974, Chapter One: "The Approach to Hematologic Problems".
Miale, Laboratory Medicine: Hematology, 4th edition, C. V. Mosby Co., St. Louis, 1972, Chapter 3, "Morphology of blood and bone marrow cells", Chapter 13, "Leukocytes and Diseases of Leukopoieses".
Ponder, Hemolysis and Related Phenomena, Second edition, Grune & Stratton, New York, 1971.
Taylor et al., J. Clin. Path., vol. 13, p. 249, 1960.
Humphries et al., Ser. Haemat., vol. V, No. 2, pp. 142-162, 1972.

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