Method for the treatment of chemonucleolysis

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases

Reexamination Certificate

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Reexamination Certificate

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07132098

ABSTRACT:
The invention relates to a method of treatment for a mammal in need of chemonucleolysis. The method comprising the administration of an effective proteoglycan cleaving amount of a proteoglycan-degrading enzyme and an effective amount of a growth factor effective in promoting the synthesis of a matrix component. The proteoglycan-degrading enzyme is preferably chondroitinase. The growth factor is preferably osteogenic protein. The proteoglycan-degrading enzyme and the growth factor are preferably administered simultaneously.

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patent: 000569541 (1995-03-01), None
patent: WO 92/13565 (1992-08-01), None
Kato, F, et al., Rinsho Seikeigeka (1987); 22 (8): 965-974. Chemonucleolysis and response of postchemonucleolytic intervertebral disc to bone morphogenetic protein BMP.
Gruber et al., J Bone Mineral Res (1996) 11 (Suppl. 1): s300. Transforming growth factor-beta 1 regulates proliferation and proteoglycan gene expression in diseased human intervertebral disc cells.
Dieudonne, et al. J of Bone and Mineral Research, 9(6), 771-780. Opposite effects of osteogenic protein and transforming growth factor-beta on chondrogenesis in cultured long bone rudiments.
Supplementary Partial European Search Report issued Oct. 20, 2004 for European Patent Application No. 99938920.8.
Kato, “Experimental Study of Chemical Spinal Fusion in the Rabbit by Means of Bone Morphogenetic Protein,” Journal of the Japanese Orthopaedic Association, vol. 64, No. 5, 1990, pp. 442-452.
Flechtenmacher, et al., “Recombinant Human Osteogenic Protein 1 is a Potent Stimulator of the Synthesis of Cartilage Proteoglycans and Collagens by Human Articular Chondrocytes,” Arthritis and Rheumatism, vol. 39, No. 11, Nov. 1996, pp. 1896-1904.
Sugimura, et al., “Experimental Chemonucleolysis with Chondroitinase ABC in Monkeys,” Spine, vol. 21, No. 2, 1996, pp. 161-165.

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