Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-02-07
1995-11-14
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 911, 435 912, 4351723, 536 231, 536 241, 935 17, 935 19, 935 20, 935 78, C07H 2100, C12N 1510, C12P 1934, C12Q 168
Patent
active
054665868
ABSTRACT:
The invention relates to a method for the synthesis of ribonucleic acid (RNA) starting from deoxyribonucleic acid (DNA). In the method of the invention, a double stranded DNA molecule is cleaved with a restriction endonuclease so as to generate a free 3'-end. The resultant DNA molecule is then denatured and hybridized with a primer that anneals to the 3'-end of the denatured DNA. The primer used in the hybridization contains a T7 promoter sequence at its 5'-end. Following the hybridization step, the 3'-ends of the resultant hybrid DNA molecule are extended with DNA polymerase to generate a template suitable for T7 RNA polymerase mediated RNA synthesis.
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Davey Cheryl
Lens Peter F.
Malek Lawrence T.
Wielaard Frits
Akzo Nobel N.V.
Gormley Mary E.
Martinell James
Stanton Brian R.
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