Method for the synthesis of DNA sequences

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S006120

Reexamination Certificate

active

10152046

ABSTRACT:
A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will spontaneously hybridize together to form the desired DNA construct. This method makes possible the remote assembly of DNA sequence, through a process analogous to facsimile transmission of documents, since the information on DNA to be made can be transmitted remotely to an instrument which can then synthesize any needed DNA sequence from the information.

REFERENCES:
patent: 5571639 (1996-11-01), Hubbell et al.
patent: 6074708 (2000-06-01), Onishi et al.
patent: 6117679 (2000-09-01), Stemmer
patent: 6218530 (2001-04-01), Rothschild et al.
patent: 6368861 (2002-04-01), Crameri et al.
patent: 6586211 (2003-07-01), Sthler et al.
patent: 6600031 (2003-07-01), Fodor et al.
patent: WO 99/42813 (1999-08-01), None
patent: WO 03/072832 (2003-09-01), None
patent: WO 03/083053 (2003-10-01), None
Dubiley et al., Nucleic Acids Research, vol. 25, pp. 2259-2265, 1997.
Peak et al., Analytical Biochemistry, vol. 169, pp. 189-193, 1988.
Sing-Gasson et al., Nature Biotechnology, vol. 17, pp. 974-978, 1999.
Merriam-Webster Online, “Overlap”, www.m-w.com/cgi-bin/dictionary?book+Dictionary&va=overlapping, last visited Jan. 12, 2006.
“Sequencing Whole Genomes,” www.bio.davidson.edu/courses/genomics/method/shotgun.html, last visited Jan. 12, 2006.
Beier et al., “Versatile derivatisation of solid support media for covalent bonding on DNA-microchips,”Nucleic Acids Research27:1970-1977 (1999).
Beier et al., “Analysis of DNA-microarrays produced by inverse in situ oligonucleotide synthesis,”Journal of Biotechnology94 15-22 (2002).
Kneidinger et al., “Scaling Up the Ligase Chain Reaction-Based Approach to Gene Synthesis,”Biotechniques30:249-252 (2001).
Au, et al., “Gene Synthesis by a LCR-Based Approach: High-Level production of Leptin-L54 Using Synthetic Gene inEscherichia coli,” Biochemical and Biophysical Research Communications248:200-203 (1998).
Beir, M., et al., “Production by quantitative photolithographic synthesis of individually quality checked DNA microarrays,” Nucleic Acids Research 28:e11i-e11vi (2000).
Blanc, A., et al., “Wavelength-Controlled Orthogonal Photolysis of Protecting Groups,” J. Org. Chem. 67:5567-5577 (2002).
Pirrung, M.C., et al., “3′-Nitrophenylpropyloxycarbonyl (NPPOC) Protecting Groups for High-Fidenity Automated 5′-3′ Photochemical DNA Synthesis,” Organic Letters 3:1105-1108 (2001).
Pon, R.T., et al., “Linker phosphoramidite reagents for oligonucleotide synthesis on underivatized solid-phase supports,” Tetrahedron Letters 42:8943-8946 (2001).
Richmond, K.E., et al., “Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis,” Nucleic Acids Research 32:5011-5018 (2004).
Shaginian, A., et al., “Light-Directed Radial Combinatorial Chemistry: Orthogonal Safety-Catch Protecting Groups for the Synthesis of Small Molecule Microarrays,” J. Am. Chem. Soc. 126:16704-16705 (2004).
Singh-Gasson, S., et al., “Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array,” Nature Biotechnology 17:974-978 (1999).
Stemmer, W.P.C., et al., “Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides,” Gene 164:49-53 (1995).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for the synthesis of DNA sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the synthesis of DNA sequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the synthesis of DNA sequences will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3871102

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.