Method for the substantial reduction of quenching effects in lum

Radiant energy – Luminophor irradiation – Methods

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250328, G01T 1204

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active

048453684

ABSTRACT:
Method for reducing quenching effects in analytical luminescence measurements. Two embodiments of the present invention are described which relate to a form of time resolution based on the amplitudes and phase shifts of modulated emission signals. In the first embodiment, the measured modulated emission signal is substantially independent of sample quenching at sufficiently high frequenices. In the second embodiment, the modulated amplitude and the phase shift between the emission signal and the excitation source are simultaneously measured. Using either method, the observed modulated amplitude may reduced to tis unquenched value.

REFERENCES:
patent: 4029401 (1977-06-01), Nather
patent: 4075480 (1978-02-01), Horrocks
patent: 4085325 (1978-04-01), Attallah et al.
patent: 4661705 (1987-04-01), Horrocks
"Enhancement of Luminescence and Raman Spectroscopy by Phase-Resolved Background Suppression", by Demas, J. N. et al., Analytical Chemistry, Feb. 1985, vol. 57, pp. 538-545.
"Elimination of Quenching Effects in Luminescence Spectrometry by Phase, Resolution", by Demans, J. N. et al., Analytical Chemistry, Jul. 1986, vol. 58, pp. 1717-1721.

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