Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1989-01-19
1991-09-24
Moskowitz, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530417, 530399, 530412, 435 691, 435 6952, C07K 322
Patent
active
050514970
ABSTRACT:
The invention relates to a method for the solubilization, by means of ion exchanger resins, of otherwise insoluble protein expressed by cultivated cells.
REFERENCES:
patent: 4757013 (1988-07-01), Inouye et al.
patent: 4766224 (1988-08-01), Rausch
Hoess et al., 1988, Bio/Technology 6:1214-1217.
Malm, B., 1987, J. Immunol. Meth. 104:103-109.
Sharma, S. K., 1986, Separation Science and Technology 21(8):701-726.
Biochem, J. (1986) 240, 1-12 (Printed in Great Britain), Review article, "The Purification of Eukaryotic Polypeptides Synthesized in Escherichia Coli", Fiona A. O. Marston.
Proc. Natl. Acad. Sci. U.S.A., vol. 84, pp. 5700-5704, Aug. 1987, Biochemistry, "Drosophila Kruppel Gene Product Produced in a Baculovirus Expression System is a Nuclear Phosphoprotin that Binds to DNA", Roger Ollo & Tom Maniatis.
"Cytoplasmic Inclusion Bodies in Escherichia coli Producing Biosynthetic Human Insulin Proteins", E. Vol. 215, Feb. 1982.
European Journal of Cell Biology 31, 171-174 (1983), "Immunocytochemical Demonstration of Human Proinsulin Chimeric Polypeptide within Cytoplasmic Inclusion Bodies of Escherichia coli", Donald C. Paul et al.
Protein Purification: Micro t Macro, pp. 429-442, "Solubilisation, Refolding and Purification of Eukaryotic Proteins Expressed in E. coli", Peter E. Lower, et al.
"The Purification of Eukaryotic Polypeptides Expressed in Escherichia coli", Fiona A. O. Marston.
Journal of Virology, Jun. 1988, p. 1999-2006, vol. 62, No. 6, copyright 1988, American Society for Microbiology "Expression of Simian Virus 40 T Antigen in Escherichia coli: Localization of T-Antigen Origin DNA-Binding Domain to Within 129 Amino Acids", Avril K. Arthur, et al.
"Molecular Mechanism of Action of the Antiobiotic Rifampicin", by Guido R. Hartmann, et al.
The Journal of Immunology copyright 1978 by The Williams & Wilkins Co., vol. 120, No. 6, Jun. 1978, "T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity", Steven Gillis, et al.
Vol. 9, No. 2, 1981, Nucleic Acids Research, "A System for Shotgun DNA Sequencing", Joachim Messing et al.
J. Mol. Biol. (1969) 41, 459-472, "A Complementation Analysis of the Restriction and Modification of DNA in Escherichia coli", Herbert W. Boyer and Daisy Roulland-Dussoix.
Proc. Natl. Acad. Sci. U.S.A., vol. 81, pp. 6647-6651, Nov. 1984, Biochemistry, "Heat Shock Regulatory Gene htpR Influences Rates of Protein Degradation and Expression of the Lon Gene in Escherichia coli", Stephen A. Goff et al.
The EMBO Journal, vol. 6, No. 9, pp. 2719-2725, 1987, "The Highly Conserved Amino-Terminal Region of the Protein Ended by the V-Myb Oncogene Functions as a DNA-Binding Domain", Karl-Heinz Klempnauer and Albert E. Sippel.
Journal of Virology, Sep. 1981, pp. 861-869, vol. 39, No. 3, "Monoclonal Antibodies Specific for Simian Virus 40 Tumor Antigens", Ed Harlow et al.
Biochemistry 1983, 22, 251-255, "Neutral Glycosphinogolipids of Murine Myeloma Cells and Helper, Cytolytic, and Suppressor T Lymphocytes", Bernhard Kniep et al.
Journal of Virology, Jul. 1987, pp. 2076-2083, vol. 61, No. 7, copyright 1987, American Society for Microbiology, "Oligomerization and Origin DNA-Binding Activity of Simian Virus 40 Large T Antigen", Robert Runzler et al.
Arthur Avril
Fanning Ellen
Hoss Adolf
Consortium fur Elektrochemische Industrie GmbH
Furman Keith L.
Moskowitz Margaret
LandOfFree
Method for the solubilization of an otherwise insoluble protein does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the solubilization of an otherwise insoluble protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the solubilization of an otherwise insoluble protein will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1697620