Method for the simultaneous dissection in specific positions...

Details Method for the simultaneous dissection in specific positions... Method for the simultaneous dissection in specific positions...

2007-08-28

2007-08-28

Moran, Marjorie A. (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Chemical analysis

C435S006120, C977S810000

Reexamination Certificate

active

10450544

ABSTRACT:
The invention relates to a method for the simultaneous dissection in specific positions of filiform organic molecular chains, in particular DNA. The aim of the invention is to provided a method, by which a highly specific dissection can take place on certain sequences that can be freely selected and simultaneously on numerous filiform molecules. To achieve this, nanoparticles (1) are provided with a molecular chain (11) of any predeterminable sequence, which is selected to be complementary to a sequence of a molecule (2) that is to be dissected, said molecular chain(s) (11) is/are hybridised in the usual manner with the molecule, or specifically linked to said molecule in another manner and the nanoparticles (1) are subsequently subjected to a high-energy radiation of at least one wavelength, which can be absorbed by said nanoparticles (1).

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Cut out or poke in—the key to the world of single genes: laser micromanipulation as a valuable tool on thelook-out for the origin of disease; 1997; Genetic Analysis Biomolecuar Engineering, by Krain Shuetze, Ingrid Becker, Karl-Friedrich et al., pp. 1-8.
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Grobunov et al. Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy; 1992; Nucleic Acids Research, vol. 20, No. 3; pp. 445-447.

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