Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1995-10-04
2000-03-07
Achutamurthy, Ponnathapura
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530412, 530416, A61K 3514, A23J 100
Patent
active
060342226
ABSTRACT:
A method for separation of recombinant pro-Factor IX from recombinant Factor IX by a chromatographic method is described. According to a preferred embodiment, the mixture of both proteins is bound to an ion exchanger and pro-Factor IX and Factor IX are eluted separately from each other by buffer solutions with different salt concentrations and/or pH values.
According to the method of the invention, pro-Factor IX and Factor IX are obtainable in highly pure form and free from the other factor respectively.
REFERENCES:
patent: 4885277 (1989-12-01), Nawroth et al.
patent: 5171569 (1992-12-01), Anson et al.
Liebman et al. (1985) Proc. Natl. Acad. Sci. USA 82, pp. 3879-3883.
Harris et al. (eds.) Protein purification methods (1989), pp. 56-66 and 202-210.
Bristol et al. (1993) J. Biol. Chem. 268, pp. 7577-7584.
Parmacia catalog (1993), pp. 328-332, 343, 344.
Tulinsky, "The Structures of Domains of Blood Proteins", Thrombosis and Hemostasis, vol. 66, No. 1, pp. 16-31 (1991).
Furie et al., "The Molecular Basis of Blood Coagulation", Cell, vol. 53, pp. 505-518, (1988).
Anderson et al., "Purification and Characterization Human Factor IX", Thrombosis Research, vol. 7, pp. 451-459, (1975).
Busby et al., "Expression of Active Human Factor IX in Transfected Cells", Nature, vol. 316 pp. 271-273, (1985).
Rees et al., "The Role of .beta.-Hydroxyaspartate and Adjacent Carboxylate Residues in the First EGF Domain of Human Factor IX", The EMBO Journal, vol. 7, No. 7, pp. 2053-2061, (1988).
Meulien et al., "Increased biological Activity of a Recombinant Factor IX Variant Carrying Alanine at Position +1", Protein Engineering, vol. 3, No. 7, pp. 629-633, (1990).
Suttie, "Current Advances in Vitamin K Research", Proceedings of the Seventeenth Steenbock Symposium, pp. 199-207, (1987).
Falkner et al., "High level Expression of Active Human Prothrombin in a Vaccina Virus Expression System", Thrombosis and Hemostasis, vol. 68, No. 2, pp. 119-124, (1992).
Barrett et al., "Large-Scale Production and Purification of a Vaccinia Recombinant-Derived HIV-1 gp 160 and Analysis of Its Immunogenicity", AIDS Res. and Human Retroviruses, vol. 5, No. 2, pp. 159-171, (1989).
Putney et al., "Large-Scale Production, Purification, and Immunologic Analysis of a Vaccinia Recombinant Derived HIV-1 gp 160", AIDS Vaccine Research and Clinical Trials, pp. 219-277, (1990).
Laemmli, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4", Nature, vol. 227, pp. 680-685, (1970).
Bradford, "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding", Analytical Biochemistry, vol. 72, pp. 248-254, (1976).
Balland et al., "Characterisation of Two Differently Processed Forms of Human Recombinant Factor IX Synth. in CHO Cells Trans. With a Polycistronic Vector", Eur. J. Biochem., vol. 172, pp. 565-572, (1988).
Kaufman et al., "Expression, Purification, and Characterization of Recombinant y-Carboxylated Factor IX Synthesized in Chinese Hamster Ovary Cells", J. Biol. Chem. vol. 261, No. 21, pp. 9622-9628, (1986).
Wasley et al., "PACE/Furin Can Process the Vitamin K-Dependent Pro-Factor IX Precursor Within the Secretory Pathway", J. Biol. Chem., vol. 268, No. 12, pp. 8458-8465, (1993).
Dorner Friedrich
Eibl Johann
Fischer Bernhard
Mitterer Artur
Achutamurthy Ponnathapura
Bio-Products & Bio-Engineering A.G.
Hildreth Ronald B.
Slobodyansky Elizabeth
LandOfFree
Method for the separation of recombinant pro-factor IX from reco does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the separation of recombinant pro-factor IX from reco, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the separation of recombinant pro-factor IX from reco will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-364358