Method for the separation of chlorophyll

Details Method for the separation of chlorophyll Method for the separation of chlorophyll

1975-10-10

1977-06-07

Jiles, Henry R.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

C07D48722

Patent

active

040282171

ABSTRACT:
To photosynthetizing living organisms such as spinach was added an organic olvent selected from the group consisting of methyl alcohol, acetone, methyl ethyl ketone and butylcarbitol in a weight amount of 3 - 8 times as much as the living organisms and the mixture is thoroughly triturated and then treated by the following route: after removal of insoluble matters, the liquid is admixed with dioxane in a volumetric amount of 1/8- 1/8 of the volume of the organic solvent and then with a phosphate buffer solution until the liquid becomes turbid, and thereafter allowed to stand in a cooled dark place, and the precipitated crystalline chlorophyll is separated from the solution.

REFERENCES:
patent: 2940981 (1960-06-01), Trurnit et al.
patent: 3274072 (1966-09-01), Burdick
patent: 3274073 (1966-09-01), Burdick
Jennison et al., "J. Tenn. Acad. Science", vol. 9, (1934), pp. 274-277.
Iriyama et al., "J. Biochemistry", vol. 76, pp. 901-904, (1974).

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