Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1980-06-17
1982-09-21
Hruskoci, Peter A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
23230B, 210635, 424 12, 435 26, G01N 3354
Patent
active
043507603
ABSTRACT:
The invention relates to a method for the selective separation of a given protein substance having an affinity for a ligand from other substances some of them having the same affinity for the ligand but also having a high molecular weight, higher than that of said protein substance, said substances being in solution.
The method consists of filtering said substances on a gel which excludes said high molecular weight substances, said gel being coupled with a ligand for which said protein substance has affinity.
The method may be applied to enzymatic and especially to immunoenzymatic assays.
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Baum et al. "Affinity Chromatography" Chapter 8, Immobilized Enzymes Antigens, Antibodies, & Peptides, Marcel Dekker Inc., N.Y., pp. 419-425.
Crastes de Paulet Andre
Descomps Bernard
Nicolas Jean-Claude
Terouanne Beatrice
Hruskoci Peter A.
Institut National de la Sante et de la Recherche Medicale
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