Method for the separation, identification and quantification of

Details Method for the separation, identification and quantification of Method for the separation, identification and quantification of

1992-01-15

1993-11-23

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, G01N 27447

Patent

active

052640981

ABSTRACT:
The invention essentially relates to a method for the separation, identification and quantification of the isoenzymes and isoforms of alkaline phosphatase in samples of physiological fluid or tissue extracts, in which method a buffer, at least one nonionic detergent and at least one anionic detergent are applied to a support medium.
Hepatic, osseous, placental, macromolecular and intestinal isoenzymes and isoenzymes bound to LpX, in particular, may be detected reliably while avoiding, as far as possible, the use of confirmation tests.

REFERENCES:
patent: 4030995 (1977-06-01), Starkweather
Biochemical Medicine, vol. 9, No. 3, Mar. 1974, pp. 309-315, N.Y., L. Fishman: "Acrylamide disc gel electrophoresis of alkaline phosphatase of human tissues, serum and ascites fluid using triton X-100 in the sample and the gel matrix".
Clinical Chemistry, vol. 28, No. 10, Oct. 1982, pp. 2007-2016, Winston-Salem, Washington, D. W. Moss et al. "Alkaline phosphatase isoenzymes".
Ari Helenius & Kai Simons "Charge shift electrophoresis: Simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution" Proceedings of the National Academy of Science USA vol. 74 No. 2 (1977) 529-532.
Ole Steen Jorgensen "Charge Shift Electrophoresis of Synaptosomal Membranes Antigens" FEBS Letters vol. 79 No. 1 (1977) 42-44.
Michele Granger-Schnarr et al "Specific Protein-DNA Complexes: Immunodetection of Protein Component after Gel Electrophoresis and Western Blotting" Analytical Biochemistry 174 (1988) 235-238.
Reinhold P. Linke, "Amphiplic Properties of the Low Molecular Weight Component of Serum Amyloid-A Protein Shown by Charge-Shift Electrophoresis" Biochimica et Biophysica Acta 668 (1984) 388-396.
Sucharit Bhakdi et al, "Detection of Amphiphilic Proteins and Peptides in Complex Mixtures, Charge-Shift Crossed Immunoelectrophoresis and Two-Dimensional Charge-Shift Electrophoresis" Biochimica et Biophysica Acta 470 (1977) 35-44.
Kunio Yokota et al, "Purification and characterization of amphiphilic trehalase from rabbit small intestine" Biochimia et Biophysica Acta 88 (1986) 405-414.

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