Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-09-19
1998-05-19
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 912, 435174, 536 231, 536 265, 536 243, 536 2432, 536 2433, C12Q 168, C12Q 170, C12P 1934, C07H 2104
Patent
active
057534330
ABSTRACT:
Method for the specific detection of nucleic acids in a sample by reaction of the sample with one or several labelled nucleotide triphosphates and one or several enzymes which catalyse the production of a labelled nucleic acid B which contains this nucleotide, non-thermally denaturation, reacting the sample with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group, if desired, contacting the nucleic acid hybrid D formed with a solid phase which recognizes and binds the immobilizable group, removing the liquid from the solid phase and determining the label on the solid phase as a measure for the presence of the nucleic acid.
REFERENCES:
patent: 5200314 (1993-04-01), Urdea et al.
patent: 5232829 (1993-08-01), Longiarn et al.
Proc. Natl. Acad. Sci. USA; 87, pp. 1874-1878 (1990) Isothermal, in-vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
Proc. Natl. Acad. Sci. USA; 86, pp. 6230-6234 (1989) Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.
Proc. Natl. Acad. Sci. USA 86, pp. 1173-1177 (1989) Transcription-based amplification system and detection of amplified human immunomodeficiency virus type 1 with a bead-based sandwich hybridization format.
Innis and Gelfand, in PCR Protocols, A Guide to Methods and Applications, pp. 3-11, Academic Press, 1990.
Berner Sibylle
Kessler Christoph
Kruse-Muller Cornelia
Ruger Rudiger
Seibl Rudolf
Boehringer Mannheim GmbH
Rees Dianne
Zitomer Stephanie W.
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