Method for the selective cleavage of fusion proteins with lysost

Details Method for the selective cleavage of fusion proteins with lysost Method for the selective cleavage of fusion proteins with lysost

1992-06-29

1993-12-14

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352573, 530412, C07K 308, C12N 1562

Patent

active

052701760

ABSTRACT:
The invention relates to a method for the preparation of polypeptides or proteins by enzymatic cleavage of the oligo- or polyglycine sequence of a fusion protein using an endoprotease.

REFERENCES:
patent: 4743679 (1988-05-01), Cohen et al.
patent: 4751180 (1988-06-01), Cousens et al.
Marston et al. Biochem. J., (1986) 240, pp. 1-12.
Iversen et al. Eur. J. Biochem., (1973) 38, pp. 293-300.
K.-K. Han et al., Int. J. Biochem. vol. 15, No. 7, pp. 875-884 (1983).
G. Sloan et al., Int. J. of Systematic Bacteriology, vol. 32, No. 2, pp. 170-174 (1982).
G. Sloan et al., Biochem. J., vol. 167, pp. 293-296 (1977).

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