Method for the selecting of genes encoding catalytic antibodies

Details Method for the selecting of genes encoding catalytic antibodies Method for the selecting of genes encoding catalytic antibodies

1991-10-22

1993-11-02

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 4351723, 424 858, C12N 1500, C12N 1513

Patent

active

052582892

ABSTRACT:
The present invention describes methods of screening for or selecting a catalytic antibody effective to cleave a target peptide. In particular, in the selection method of the present invention a phage gene is selected that encodes a gene product necessary for the production of a phage. The phage, carrying the modified gene is introduced into a host. Also, a library of rearranged immunoglobulin genes in a cloning vector is introduced into host cells. The host cells are grown under conditions in which the immunoglobulin genes are expressed in the host cells. The presence of antibodies capable of cleaving the target peptide is identified on the basis of production of phage.

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