Method for the screening, the detection and/or the...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S006120

Reexamination Certificate

active

09816763

ABSTRACT:
The present invention is related to a screening, detection and/or quantification method of one or more transcriptional factor(s) (1) possibly present in a biological sample, said method comprising the steps of:possibly extracting and isolating said transcriptional factor (1) from said biological sample,putting into contact the transcriptional factor (1) with a double-stranded DNA sequence (2) bound to an insoluble solid support (3), anddetecting and/or quantifying said fixed transcriptional factor (1),said double-stranded DNA sequence having a specific sequence able to be fixed by the transcriptional factor (1) and being preferably located at a distance of at least about 6.8 nm from the surface of the solid support (3), and said double-stranded DNA sequence being bound to the surface of the insoluble solid support (3) at a concentration of at least 0.01 pmole/cm2of solid support surface (3).The present invention is also related to the kit comprising means and media for performing said method.

REFERENCES:
patent: 4968602 (1990-11-01), Dattagupta
patent: 5563036 (1996-10-01), Peterson et al.
patent: 5747253 (1998-05-01), Ecker et al.
patent: 5770722 (1998-06-01), Lockhart et al.
patent: 5846683 (1998-12-01), Murakami et al.
patent: 5939261 (1999-08-01), Loewy et al.
patent: 5976795 (1999-11-01), Voytas et al.
patent: 6326489 (2001-12-01), Church et al.
patent: 6342353 (2002-01-01), Heslot et al.
patent: 2002/0115198 (2002-08-01), Nerenberg et al.
patent: 0620439 (1994-04-01), None
patent: WO 89/11548 (1989-11-01), None
patent: WO 95/30026 (1995-11-01), None
patent: WO 98/03652 (1998-01-01), None
patent: WO 98/08096 (1998-02-01), None
patent: WO 00/22167 (2000-04-01), None
Brand et al., “Activated Transcription FActor Nuclear Factor-Kapa B is Present in the Atherosclerotic Lesion,” Journal of Clinical Investigation, APr. 1996, vol. 97, No. 7, pp. 1715-1722.
Guo, Z., et al., (1994) Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acids Research 22:5456-5465.
Hibma, M., et al., (1994) A non-radioactive assay for the detection and quantitation of a DNA binding protein. Nucleic Acids Research 22:3806-3807.
Anthony, R.M., et al., (2000) Rapid Diagnosis of Bacteremia by Universal Amplification of 23S Ribosomal DNA Followed by Hybridization to an Oligonucleotide Array. J. Clinical Microbio. (No. 2) 38: 781-788.
Zammatteo, N., et al., (1997) Comparison between microwell and bead supports for the detection of human cytomegalovirus amplicons by sandwich hybridization. Anal. Biochem. 253: 180-189.
European Search Report, from Priority Application EP 00870057 dated Sep. 14, 2000.
Baeuerle et al., Advances in Immunology,NF-κB as a Frequent Target for Immunosuppressive and Anti-Inflammatory Molecules, vol. 65 (1997), pp. 111-137.
Benotmane et al., Analytical Biochemistry,Nonisotopic Quantitative Analysis of Protein-DNA Interactions at Equilibrium, vol. 250, (1997) pp. 181-185.
Gubler et al., BioTechniques,Nonradioactive Assay for Sequence-Specific DNA Binding Proteins, vol. 18, No. 6 (1995) pp. 1008, 1011-1014.
Schreck et al., Nucleic Acids Research,The NF-κB transcription factor induces DNA bending which is modulated by its 65-kD subunit, vol. 18, No. 22 (1990), pp. 6497-6502.
Yi et al., Biochemistry,Divalent Cations Stimulate Preferential Recognition of a Viral DNA End by HIV-1 Integrase, vol. 38, No. 26 (1999), pp. 8458-8468.
Zabel et al., EMBO Journal,Nuclear uptake control of NF-κB by MAD-3, an IκB protein present in the nucleus, vol. 12, No. 1 (1993) pp. 201-211.
Bielinska, A. et al., “Regulation of Gene Expression with Double-Stranded Phosphorothioate Oligonucleotides,”Science250:997-1000 (1990).
Brivanlou, A. et al., “Signal Transduction and the Control of Gene Expression,” Science, 295:813-818 (2002).
Ghosh, I. et al., “Structure-Function Relationship in a 3-Sheet Peptide Inhibitor of E47 Dimerization and DNA Binding,”Bioorg.&Med. Chem. 7:61-66 (1999).
Grigoriev, M. et al., “Inhibition of Gene Expression by Triple Helix-Directed DNA Cross-Linking at Specific Sites,”PNAS USA90:3501-3505 (1993).
Nielsen, P. et al., “Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide,”Science254:1497-1500 (1991).
Tanaka, H. et al., “Sequence-specific interaction of a ∀∃-anomeric double-stranded DNA with the p50 subunit of NFιB: application to the decoy approach,”Nucleic Acids Research22:3069-3074 (1994).
Yao, S. et al., “Uncoiling c-Jun Coiled Coils: Inhibitory Effects of Truncated Fos Peptides on Jun Dimerization and DNA Binding In Vitro,”Biopolymerics47:277-283 (1998).
Ghiorzo, P. et al. (1997) “c-Rel and p65 subunits bind to an upstream NF-κB site in human granulocyte macrophage-colony stimulating factor promoter involved in phorbol ester response in 5637 cells”FEBS Letters418:215-218.
Kaltschmidt, C. et al. (1995) “Selective recognition of the activated for of transcription factor NF-xB by a monoclonal antibody”Biol. Chem. Hoppo-Seyler376:9-16.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for the screening, the detection and/or the... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the screening, the detection and/or the..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the screening, the detection and/or the... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3905946

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.