Method for the reduction of heterogeneity of monoclonal antibodi

Details Method for the reduction of heterogeneity of monoclonal antibodi Method for the reduction of heterogeneity of monoclonal antibodi

1989-06-09

1992-06-30

Rosen, Sam

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 691, 435 695, 435272, 43524027, 530402, 5303881, 5303873, 53038815, C12P 2106, C12P 2108

Patent

active

051262509

ABSTRACT:
Novel methods for reducing the heterogeneity of secreted monoclonal antibodies are disclosed. The first method comprises incubating the heterogeneous antibodies at low pH for a length of time sufficient to convert the heterogeneous forms of antibodies into substantially homogeneous forms. Another method produces the same result using ascites fluid, while yet another method produces the same result using carboxypeptidase. The homogeneous antibodies can then be purified in high yield.

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McDonough et al., 1989, Abstract and Data from a Poster presented at the UCLA Symposium on Protein Engineering, Jan. 17 through Jan. 22.
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