Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1980-03-21
1981-12-22
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 435 15, 435 29, 424 1, 424 7, 23230B, C12Q 168, C12Q 148, C12Q 129, C12Q 100
Patent
active
043071899
ABSTRACT:
Terminal Deoxynucleotidyl Transferase (TdT) may now be quantified in a biological sample without interference from other DNA polymerases normally found therein by utilizing labeled deoxynucleoside triphosphates and/or labeled single stranded oligodeoxynucleotides which have been chemically modified to impair or destroy their ability to form nucleotide base pairs by hydrogen bonding. Such modified deoxynucleoside triphosphates and/or oligodeoxynucleotides of impaired base forming abilities are selectively synthesized by TdT into fluorescently or radioactively labeled acid insoluble polydeoxynucleotides which may be quantified to determine the amount of TdT originally present in the biological sample.
REFERENCES:
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