Method for the quantitative determination of physiological compo

Details Method for the quantitative determination of physiological compo Method for the quantitative determination of physiological compo

1985-06-27

1989-06-13

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

436 12, 436 63, 436811, 436815, C12Q 125, G01N 3348

Patent

active

048392790

ABSTRACT:
A novel enzyme of bilirubin oxidase and a conventional enzyme of laccase are found to oxidize bilirubin to biliverdin without formation of hydrogen peroxide, thereby permitting the content of conjugated bilirubin in biological fluid to be quantitatively determined by reacting therewith the bilirubin oxidase of a microorganism of genus Myrothecium origin, and also permitting the content of total bilirubin in biological fluid to be quantitatively determined by reacting therewith such a bilirubin oxidase or laccase in the presence of a specific additive compound.

REFERENCES:
patent: 4211844 (1980-07-01), Wu
patent: 4571383 (1986-02-01), Takayama et al.
patent: 4600689 (1986-07-01), Matsui et al.
patent: 4701411 (1987-10-01), Wu
Murao, S et al, "A New Enzyme `Bilirubin Oxidase` . . . " Agric. Biol. Chem. 45(10), 2383-2384 (1981).
Nakamura, T. "Purification and Physico-Chemical Properties of Laccase", Biochem. Biophys. Acta 30, 44-52 (1980).

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