Method for the quantitative determination of calcium in biologic

Details Method for the quantitative determination of calcium in biologic Method for the quantitative determination of calcium in biologic

1985-02-21

1986-10-21

Kaplan, G. L.

Chemistry: analytical and immunological testing

Metal or metal containing

Present in biological fluids

73 1R, 204 1T, 204409, 436 8, 436 79, 436 16, G01N 2746, G01N 3348

Patent

active

046185879

ABSTRACT:
A method is disclosed for determining calcium ion content of serum which comprises calibrating a potentiometric analytical system with calcium standards having a solution containing both TRIS and BisTRIS at a pH of 7.5. The serum sample is diluted with a silicone oil/water emulsion which may further contain a protein complexing agent such as iron, zinc, cadmium, nickel or copper. Measurement is then carried out on the diluted serum sample.

REFERENCES:
patent: 3932233 (1976-01-01), Ruzicka et al.
patent: 3957607 (1976-05-01), Simon et al.
patent: 4410631 (1983-10-01), Czaban et al.
J. D. R. Thomas, "Ion-Selective Electrode Reviews", vol. 3, p. 192, (1982).
Henry Freiser, "Ion-Selective Electrodes in Analytical Chemistry", vol. I, pp. 272-278, (1978).
Arthur K. Covington, "Ion-Selective Electrode Methodology", vol. II, pp. 55 & 56, (1980).
L. Keil et al., Analyst, 102, 274, (1977).

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