Method for the quantitative detection of nucleic acids

Details Method for the quantitative detection of nucleic acids Method for the quantitative detection of nucleic acids

2006-04-18

2006-04-18

Fredman, Jeffrey (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S091200, C536S022100, C536S023100, C536S024300, C536S024310

Reexamination Certificate

active

07029843

ABSTRACT:
Provided herein is a method for the quantitative detection of nucleic acids based on the use of a calibrator, suitable primers and probes, and a nucleic acid polymerase with 5′-3′ nuclease activity.

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Gibson U. E. M. et al.: “A Novel Method for Real Time Quantitative RT-PCR” Genome Research, US, Cold Spring Harbor Laboratory Press, vol. 6, No. 10, Oct. 1996, pp. 995-1001.
Woudenberg T. M. et al.: “Quantitative PCR by Real Time Detection” Proceedings Of The SPIE, vol. 2680, Jan. 1996.
Secchiero P. et al.: “Quantitative PCR for Human Herpesviruses 6 and 7” Journal Of Clinical Microbiology, US, Washington, DC, vol. 33, No. 8, Aug. 1995, pp. 2124-2130.
Kennedy M. M. et al.: “Identification of HHV8 in Early Kaposi's Sarcoma: Implications For Kaposi's Sarcoma Pathogenesis” Molecular Pathology, vol. 51, No. 1, Feb. 1998, pp. 14-20.
Zimmermann K. et al.: “Technical Aspects of Quantitative Competitive PCR” Biotechniques, US, Eaton Publishing, Natick, vol. 21, No. 2, Aug. 1996, pp. 268-270, 272, 27.

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