Method for the purification of LPF-HA

Details Method for the purification of LPF-HA Method for the purification of LPF-HA

1987-11-16

1989-12-05

Stone, Jacqueline M.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Glycoprotein, e.g., mucins, proteoglycans, etc.

530413, 530825, 514 2, 424 88, 424 92, 424 95, 435 713, 435803, C07K 1504, C07K 1712, A61K 3702, A61K 3910

Patent

active

048853593

ABSTRACT:
Improved method for the purification of LPF-HA (Leucocytosis promoting far hemagglutinin) on industrial scale which comprises contacting a LPF-HA-containing solution from culture media of Bordetella pertussis with a cellulose sulfate gel, a crosslinked polysaccharide sulfate gel, or a polysaccharide gel chemically bound with dextran sulfate, thereby adsorbing LPF-HA on the gel, and then eluting LPF-HA from the gel. Said method can give a highly purified LPF-HA which does not contain any other proteins, lipid, saccharides, etc. and further undesirable endotoxin, and hence can be used for producing various reagents, medicines and pertussis vaccine.

REFERENCES:
patent: 3184394 (1965-05-01), Schmidtberger et al.
patent: 4022758 (1977-05-01), Andersson et al.
patent: 4247452 (1981-01-01), Irons et al.
patent: 4515714 (1985-05-01), Kawahara et al.
patent: 4563303 (1986-01-01), Ginnaga et al.
patent: 4687738 (1987-08-01), Ginnaga et al.
patent: 4704274 (1987-11-01), Sakuma et al.

Affiliated with

Also associated with

Rating

Method for the purification of LPF-HA does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the purification of LPF-HA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the purification of LPF-HA will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2035162