Method for the purification of LPF-HA

Drug – bio-affecting and body treating compositions – Lymphokine

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424 92, 435 68, 435 70, 435822, 530413, A61K 3902, C07K 320

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active

047042745

ABSTRACT:
A method for producing highly pure LPF-HA (Leucocytosis-promoting Factor agglutinin) from culture media of Bordetella pertussis in a high yield by an affinity chromatography using a denatured ceruloplasmin as a ligand. The LPF-HA obtained by this invention is highly pure and does not contain almost endotoxin of B. pertussis and is useful for the preparation of various reagents utilizing the physiological activities, a medicine for treating diabetes, and a pertussis vaccine.

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patent: 4551429 (1985-11-01), Greenspan
patent: 4563303 (1986-01-01), Gennaga et al.
Science, 127, 1963, p. 588, Morell et al.
Infect and Immunity, 41(1), 1983, pp. 313-320, Sato et al.
Infect and Immunity, 39(2), 1983, pp. 590-598, Robinson et al.
C. A., vol. 96, 1982, #197728e, Cowell et al.

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