Method for the purification of interferon

Details Method for the purification of interferon Method for the purification of interferon

1980-10-17

1982-11-16

Adee, John

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 424 85, 435811, 260112R, B01D 1508

Patent

active

043593895

ABSTRACT:
Human fibroblast interferon may be purified to a high degree by using a simple two-step purification method comprising (a) subjecting an aqueous interferon solution to chromatography on porous glass beads, and (b) subjecting the resulting aqueous interferon solution to chromatography on immobilized zinc chelate. Overall recoveries of about 45-76% of the initial interferon activity may be achieved and the end product will be free of any skin reactive agents.

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