Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1989-03-09
1991-09-10
Moskowitz, Margaret
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530413, 530412, 530415, C07K 318, C07K 328, C07K 1506
Patent
active
050475061
ABSTRACT:
The invention relates to a method for the purification of the a subunit of factor XIII by affinity chromatography, to a therapeutic composition containing the latter, and to the use of the therapeutic composition.
Factor XIII has hitherto been purified either by methods which are technically very elaborate or else by use of toxic affinity chromatography materials. The invention has the aim of providing an improved method for the purification of the a subunit of factor XIII.
Factor XIII is obtained according to the invention by a method in which the a subunit of factor XIII is reversibly bound to a matrix suitable for disulfide exchange reactions and is removed from the matrix by reaction with a reducing agent. The method according to the invention makes it possible to provide the biologically active a subunit of factor XIII in high purity.
REFERENCES:
patent: 4237267 (1980-12-01), Okuyama et al.
patent: 4828989 (1989-05-01), Prior et al.
Abstract, Dialog File 72, Embase No: 87014291 of Grundman et al., 1986, Proc. Natl. Acad. Sci. U.S.A. 83(21): 8024-8028.
Biochimica et Biophysica Acta, "Affinity Chromatography of Human Plasma and Platelet Factor XIII on Organomercurial Agarose", J. McDonagh et al., vol. 446, pp. 345-357 (1976).
Biochemistry, "Calcium-Dependent Unmasking of Active Center Cysteine During Activation of Fibrin Stabilizing Factor", C. G. Curtis et al., vol. 13, No. 18, pp. 3774-3780 (1974).
Lobermann Hartmut
Romisch Jurgen
Stuber Werner
Behringwerke Aktiengesellschaft
Furman Keith C.
Moskowitz Margaret
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