Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1985-01-11
1987-06-30
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
424 88, 424 99, 435 68, 514 8, 530380, 530834, C07K 1514, C07K 320, A61K 3522, A61K 3724
Patent
active
046771956
ABSTRACT:
A method for purifying erythropoietin is described. The method comprises treating partially purifying erythropoietin by reverse phase high performance liquid chromatography to obtain homogeneous erythropoietin having a molecular weight of about 34,000 daltons on SDS PAGE and moving a single peak on reverse phase HPLC. The homogeneous erythropoietin protein preferably has a specific activity of at least 120,000 IU, more preferably at least 160,000 IU per absorbance unit at 280 nm.
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Hewick Rodney M.
Seehra Jasbir S.
Berstein David L.
Eisen Bruce M.
Genetics Institute Inc.
Schain Howard E.
Solomon Teresa L.
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