Method for the purification of collagens

Details Method for the purification of collagens Method for the purification of collagens

1981-10-19

1983-07-05

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

260112B, A61K 3516, C07G 700, C08H 106, C09H 100

Patent

active

043917496

ABSTRACT:
Collagen affinity matrices are prepared, useful for separating collagenous substances from other high molecular weight proteinaceous substances. Fibronectin is fragmentated chemically or by enzymatic digestion, etc. Fragments of fibronectin, which retain the collagen-binding site but are severed from other binding sites of intact fibronectin, are selected and coupled to solid support material.

REFERENCES:
patent: 4104266 (1978-08-01), Wickerhauser
patent: 4210580 (1980-07-01), Amrani
patent: 4341764 (1982-07-01), Wallace et al.
Engvall et al., Int. J. Cancer: 20, 1-5, (1977).
Ruoslahti et al., Biochimica et Biophysica Acta, 534 (1978), 210-218.
Ruoslahti et al., Coll. Res., vol. 1, (1981), 95-128.

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