Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2011-08-09
2011-08-09
Saucier, Sandra (Department: 1651)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S195000, C424S094630, C424S094640
Reexamination Certificate
active
07993899
ABSTRACT:
We describe a method for purifying chymosin comprising providing an aqueous liquid sample containing chymosin and a separation medium comprising a base matrix and a plurality of firmly attached ligands that are capable of binding to chymosin, contacting the matrix with the sample under conditions permitting binding of chymosin to the matrix, and desorbing chymosin from the matrix. The characterizing feature is that the matrix is hydrophilic and that the ligands in the plurality of ligands are hydrocarbon groups in which all carbon atoms are sp3-hybridised, possibly with an ether oxygen or a thioether sulphur inserted between two carbon atoms at one or more positions in at least one of the hydrocarbon groups, and possibly a hydroxy group replacing a hydrogen atom at one or more positions in at least one of the hydrocarbon groups.
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Caussette Mylene
Fährenmark Johan
Lagerlund Inger
Morenweiser Robert
Piron Eric
DSM IP Assets BV
GE Healthcare Bio-Sciences AB
Hanley Susan
Nixon & Vanderhye PC
Saucier Sandra
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