Method for the purification of an N-terminal fragment of...

Details Method for the purification of an N-terminal fragment of... Method for the purification of an N-terminal fragment of...

2005-03-02

2009-06-09

Allen, Marianne P (Department: 1647)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

C435S071200, C435S320100, C435S325000, C435S243000, C435S259000, C530S412000

Reexamination Certificate

active

07544493

ABSTRACT:
A method for the production of the N-terminal four kringle-containing fragment of hepatocyte growth factor (NK4) by expression of a nucleic acid encoding said NK4 in a microbial host cell, isolation of inclusion bodies containing said NK4 in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK4, characterized in that solubilization and naturation are performed at pH 7-9 in phosphate buffered solution, provides NK4 in high purity and high yield.

REFERENCES:
patent: 5977310 (1999-11-01), Namiki et al.
patent: 6855685 (2005-02-01), Nakamura
patent: 2004/0052777 (2004-03-01), Nesbit et al.
patent: 1 234 583 (2002-08-01), None
patent: WO 93/23541 (1993-11-01), None
Date et al., FEBS Letters, 420, pp. 1-6 (1997).
Albini et al., Cancer Research, 47, pp. 3239-3245 (1987).
Date et al., Oncogene, 17, pp. 3045-3054 (1998).
Kuba et al., Cancer Research, 60, pp. 6737-6743 (2000).
Miyazawa et al., Biochem. Biophys. Res. Comm., 163, pp. 967-973 (1989).
Nakamura et al., Biochem. Biophys. Res. Comm., 122, pp. 1450-1459 (1984).
Nakamura et al., Nature, 342, pp. 440-443 (1989).
Okajima et al, Eur. J. Biochem, 193, pp. 375-381 (1990).
Parr et al., Int. J. Cancer, 85, pp. 563-570 (2000).
Seki et al., Biochem. Biophys. Res. Comm., 172, pp. 321-327 (1990).
Stahl et al., Biochem. J., 326, pp. 763-772 (1997).
Stuart et al., Int. J. Exp. Path., 81, pp. 17-30 (2000).
Tashiro et al., Proc. Natl. Acad. Sci. USA, 87, pp. 3200-3204 (1990).
Weidner et al., Proc. Natl. Acad. Sci. USA, 88, pp. 7001-7005 (1991).
Brinkmann et al., Gene, 85, pp. 109-114 (1989).
Kuba et al., Biochem. Biophys. Comm., 279, pp. 846-852 (2000).
Hirao et al., Cancer Gene Therapy, 9, pp. 700-7007 (2002).

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