Method for the production of polypeptides

Details Method for the production of polypeptides Method for the production of polypeptides

1997-07-07

2000-08-29

Schwartzman, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 694, 4352551, 4352542, 435471, 536 231, C12P 2100, C12N 1574, C12N 1580, C07H 2104

Patent

active

061107034

ABSTRACT:
The present invention relates to a novel method for the production of short chain polypeptides, including polypeptides having up to 3 disulfide bonds and/or structures rich in basic amino acid residues, and open structured short chain polypeptides, e.g. glucagon, glucagon like peptides and their functional analogues, in genetically modified yeast cells, said genetically modified yeast cells, and a method for the preparation of said yeast cells.

REFERENCES:
patent: 5179003 (1993-01-01), Wolf et al.
patent: 5679544 (1997-10-01), Fleer et al.
Azaryan et al. Characterisics of Yap3, a new prohormone processing aspartic protease from S. cerevisiae. Acv. Exp. Med. and Biol. vol. 362:569-572, Dec. 1995.
Komano et al. Shared functions in vivo of a glycosyl-phosphatidylinositol-linked aspartyl protease, Mkc7, and the proprotein processing protease Kex2 in yeast. PNAS Vo. 92:10752-10756, Nov. 1995.
Gabrielsen et al., (1990) Gene 90:255-262.
Egel-Mitani et al., (1990) Yeast 6:127-137.

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