Method for the production of phenylalanine ammonia-lyase by ferm

Details Method for the production of phenylalanine ammonia-lyase by ferm Method for the production of phenylalanine ammonia-lyase by ferm

1983-10-31

1986-04-22

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435108, 435801, C12N 988, C12P 1322

Patent

active

045842736

ABSTRACT:
A method for the production of the enzyme, phenylalanine ammonia-lyase (PAL), by fermentation, wherein the stability and useful life of the enzyme are improved by maintaining PAL-containing media in substantially anaerobic, static conditions. PAL is useful for catalyzing the conversion of t-cinnamic acid and ammonia to L-phenylalanine.

REFERENCES:
patent: 3791924 (1974-02-01), Ogata et al.
patent: 3917511 (1975-11-01), Nakayama et al.
patent: 3957580 (1976-05-01), Nelson
patent: 4375515 (1983-03-01), Patel et al.
"L-Phenylalanine Ammonia-Lyase, II, Mechanism and Kinetic Properties of the Enzyme from Potato Tubers," by Havir and Hanson, Biochemistry vol. 7, No. 5, May 1968.

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