Method for the production of HA fraction containing protective a

Details Method for the production of HA fraction containing protective a Method for the production of HA fraction containing protective a

1986-06-16

1987-08-18

Kight, John

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 3, 435802, 435253, 435822, 435812, 435813, 424 92, 424 88, 424 93, C12P 2100

Patent

active

046877380

ABSTRACT:
A method for production of HA fraction containing protective antigens of Bordetella pertussis (i.e. a fraction containing F-HA and LPF-HA) in an industrial scale, for instance, with a fermentater, which comprises inoculating a strain of B. pertussis in a liquid culture containing a cyclodextrin or its derivative, culturing it by a spinner culture under controlling the culture temperature and the amount of dissolved oxygen and under defoaming condition, optionally under controlling the pH range, and harvesting the produced HA fraction from the culture broth at a stage of from logarithmic growth phase to static grow phase, and a method for production of a pertussis vaccine from the HA fraction thus obtained by formalinizing the HA fraction in the presence of an amino acid.

REFERENCES:
patent: 3577319 (1971-05-01), Nielsen et al.
patent: 4468455 (1984-08-01), Hopkins
patent: 4500639 (1985-02-01), Suzuki et al.
patent: 4551429 (1985-11-01), Greenspan
patent: 4563303 (1986-01-01), Ginnoya et al.
Imaizumi et al, Inf. and Immun, 41, 1983, pp. 1183-43, "Effect . . . Cyclodextran on the Production of Toxin by B. pertussis".
Imaizumi et al, "Heptakis(2,6-O-dimethyl).beta.-cyclodextran", J. Clin. Microbiol., 17(15), 1983, pp. 781-786.
Imaizumi et al, "A New Culturing Method . . . B. pertussis", J. Microbiol. Methods, 2(6), 1984, pp. 339-348.
Stainir et al, "A Simple Chemically Defined Medium . . . B. pertussis", J. Gen. Microbiol., 63, 1971, pp. 211-220.

Affiliated with

Also associated with

Rating

Method for the production of HA fraction containing protective a does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the production of HA fraction containing protective a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the production of HA fraction containing protective a will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1117814