Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2007-08-28
2007-08-28
Kam, Chih-Min (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S488000, C435S252330, C536S023200
Reexamination Certificate
active
10336844
ABSTRACT:
This invention relates to a method for the production of D-(−)-3-hydroxybutyric acid, comprising the step of culturing a recombinant strain containing genes phbA, phbB, ptb and buk by fermentation. Preferably, the recombinant strain is a strain ofE. coli.The method of the invention is simple, avoiding the technique of degrading polymer to produce D-(−)-3-hydroxybutyric acid. The present method also provides improved efficiency, lowers the complicated requirement for facilities as used in traditional chemical synthesis, simplifies the complicated technique flow, and omits the complicated chiral separation step. Therefore, the present method greatly reduces the costs associated with D-(−)-3-hydroxybutyric acid production. Also, with this invention, the problems such as environmental pollution of chemical synthesis and chiral separation are overcome.
REFERENCES:
patent: 2096035 (1997-11-01), None
patent: WO 00/28985 (2000-05-01), None
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Putnoky, P., A. Kereszt, T. Nakamura, G. Endre, E. Grosskopf, P. Kiss, and A. Kondorosi. 1998. The pha gene cluster ofRhizobium melilotiinvolved in pH adaptation and symbiosis encodes a novel type of K+ efflux system. Mol Microbiol. 28:1091-101.
GenBank Accession No. AF078795. Dec. 9, 1998.Alcaligenes latuspolyhydroxyalkanoate synthase protein PhaC (phaC), ketothiolase protein PhaA (phaA), and reductase protein PhaB (phaB) genes, complete cds.
GenBank Accession No. J04987. Apr. 24, 1993. A.eutrophus beta-ketothiolase (phbA) and acetoacetyl-CoA reductase (phbB) genes, complete cds.
GenBank Accession No. L01112. Apr. 26, 1993.Chromatium vinosumpoly(3-hydroxybutyric acid) phbA, phbB, phbC) genes, complete cds.
GenBank Accession No. U47026. Jan. 29, 1999.Alcaligens latuspoly(3-hydroxyalkanoate) synthesis (phaC, phaA, and phaB) genes, complete cds.
GenBank Accession No. X93358. Mar, 12, 1999.Rhizobium melilotipha[A,B,C,D,E,F,G] genes.
GenBank Accession No. L01113. Jun. 26, 1996.Thiocystis violaceabeta-ketothiolase and PHA synthase (phbA and phbC) genes, complete cds.
Chen Guoqiang
Chen Jinchun
Gao Haijun
Dykema Gossett PLLC
Kam Chih-Min
Zinkl Gregory M.
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