Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Bacterium or component thereof or substance produced by said...
Patent
1999-12-15
2000-12-19
Chan, Christina Y.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Bacterium or component thereof or substance produced by said...
424803, 530853, 5303895, A61K 3909, A61K 3940, B28B 1700, C07K 1600
Patent
active
061624411
ABSTRACT:
Disclosed is a method for producing anti-E. coli O157 antibodies. Anti-E. coli O157:H7 antibodies are produced in egg-laying hens and isolated from the eggs. E. coli O157:H7 is cultured in a brain heart infusion broth and killed by the treatment with hot water for 5-10 min. After being collected by centrifugation, the dead bacteria is homogenized. Serving as an antigenic material, the bacterial homogenate is injected into egg-laying hens to induce antibodies against E. coli O157:H7 in the eggs. The induced antibodies are isolated from the yolk of the eggs and the eggs containing the antibodies against E. coli O157:H7 can be utilized, in their entirety, for foods. Alternatively, the yolks are separated from the eggs and freeze-dried. The resulting dried egg component can be applied to processed foodstuffs, alone or in combination with whole eggs. Since the antibodies against E. coli O157:H7 are contained in frozen eggs, it is very convenient to store the antibodies. The storage in the frozen eggs also makes it possible to apply the antibodies to almost all foods at any time.
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Ahn Chong-Nam
Chae Hyun-Seok
Cho Sung-Geun
Kim Dong-Woon
Kim Yong-Gon
Chan Christina Y.
Ewoldt Gerald R.
Republic of Korea (Management: Rural Development Administration)
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