Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a...
Reexamination Certificate
2003-01-22
2009-10-27
Wang, Andrew (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing a...
C435S189000
Reexamination Certificate
active
07608421
ABSTRACT:
The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
REFERENCES:
patent: 5480805 (1996-01-01), Wolf et al.
patent: 486290 (1992-05-01), None
patent: WO-98/45457 (1998-10-01), None
patent: WO-99/16886 (1999-04-01), None
patent: WO-02/061072 (2002-08-01), None
Arthington, et al. “Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase.”Gene. Jun. 15, 1991; 102(1):39-44.
Ashman, et al. “Cloning and disruption of the yeast C-8 sterol isomerase gene.”Lipids. Aug. 1991; 26(8):628-32.
Avruch, et al. “The induced biosynthesis of 7-dehydrocholesterols in yeast: potential sources of new provitamin D3analogs.”Can J Biochem. Jul. 1976; 54(7):657-65.
Basson, et al. “Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate-limiting enzyme of sterol biosynthesis.”Mol Cell Biol. Sep. 1988; 8(9):3797-808.
Braverman, et al. “Mutations in the gene encoding 3 β-hydroxysteroid-Δ8, Δ7-isomerase cause X-linked dominant Conradi-Hunermann syndrome.”Nat Genet. Jul. 1999; 22(3):291-4.
Chang, et al. “Molecular cloning and functional expression of human acyl-coenzyme A:cholesterol acyltransferase cDNA in mutant Chinese hamster ovary cells.”J Biol Chem. Oct. 5, 1993; 268(28):20747-55.
Hardwick, et al. “SED6is identical toERG6, and encodes a putative methyltransferase required for ergosterol synthesis.”Yeast. Feb. 1994; 10(2):265-9.
Husselstein, et al. “Δ7-sterol-C5-desaturase: molecular characterization and functional expression of wild-type and mutant alleles.”Plant Mol Biol. Mar. 1999; 39(5):891-906.
Jandrositz, et al. “The gene encoding squalene epoxidase fromSaccharomyces cerevisiae: cloning and characterization.”Gene. Oct. 30, 1991: 107(1):155-60.
Jennings, et al. “Molecular cloning and characterization of the yeast gene for squalene synthetase.”Proc Natl Acad Sci USA. Jul. 15, 1991; 88(14):6038-42.
Kalb, et al. “Isolation of a cytochrome P-450 structural gene fromSaccharomyces cerevisiae.” Gene. 1986; 45(3):237-45.
Lai, et al. “The identification of a gene family in theSaccharomyces cerevisiaeergosterol biosynthesis pathway.”Gene. Mar. 11, 1994; 140(1):41-9.
Lees, et al. “Cloning of the late genes in the ergosterol biosynthetic pathway ofSaccharomyces cerevisiae—a review.”Lipids. Mar. 1995; 30(3):221-6.
Nishi, et al. “cDNA cloning of the mammalian sterol C5-desaturase and the expression in yeast mutant.”Biochim Biophys Acta. Jan. 31, 2000; 1490(1-2):106-8.
Polakowski, et al. “Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast.”Appl Microbiol Biotechnol. Jan. 1998; 49(1):66-71.
Silve, et al. “Emopamil-binding protein, a mammalian protein that binds a series of structurally diverse neuroprotective agents, exhibits Δ8-Δ7sterol isomerase activity in yeast.”J Biol Chem. Sep. 13, 1996; 271(37):22434-40.
Skaggs, et al. “Cloning and characterization of theSaccharomyces cerevisiaeC-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.”Gene. Feb. 22, 1996; 169(1):105-9.
Tainaka, et al. “Effects of elevated expression of theCYP51(P45014DM) gene on the sterol contents ofSaccharomyces cerevisiae.” Journal of Fermentation and Bioengineering1995; 79(1):64-6.
Uelmen, et al. “Tissue-specific expression and cholesterol regulation of acylcoenzyme A:cholesterol acyltransferase (ACAT) in mice. Molecular cloning of mouse ACAT cDNA, chromosomal localization, and regulation of ACAT in vivo and in vitro.”J Biol Chem. Nov. 3, 1995: 270(44):26192-201.
Waterham, et al. “Mutations in the 3β-hydroxysterol Δ24-reductase gene cause desmosterolosis, an autosomal recessive disorder of cholesterol biosynthesis.”Am J Hum Genet. Oct. 2001; 69(4):685-94.
Xu, et al. “Biosynthesis of cholesterol in the yeast mutant erg6.”Biochem Biophys Res Commun. Aug. 30, 1988; 155(1):509-17.
Yang, et al. “Sterol esterification in yeast: a two-gene process.”Science. May 31, 1996; 272(5266): 1353-6.
Yu, et al., “Molecular Cloning and Characterization of Two lsoforms ofSaccharomyces cerevisiaeAcyl-CoA:Sterol-Acyltransferase.”J. Biol. Chem. Sep. 27, 1996; 271(39): 24157-63.
T. Polakowski, Molekularbiologische Beeinflussung des Ergosterolstoffwechsels der HefeSaccharomyces cerevisiae, Shaker Verlag Aachen, 1999, Seite 59 bis 66.
International Search Report for PCT/EP 03/00592 (4 pages).
Pena-Diaz, J. et al., “A soluble 3-hydroxy-3-methylglutaryl-CoA reductase in the protozoanTrypanosoma cruzi”, Biochem. J., (1997), vol. 324, pp. 619-626.
Basson, M.E. et al., “Structural and Functional Conservation between Yeast and Human 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductases, the Rate-Limiting Enzyme of Sterol Biosynthesis”, Molecular and Cellular Biology, Sep. 1988, vol. 8, No. 9, pp. 3797-3808.
Bischoff, K.M. et al., “3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase fromHaloferax volcanii: Purification, Characterization, and Expression inEscherichia coli”, Journal of Bacteriology, Jan. 1996, vol. 178., No. 1, pp. 19-23.
Bochar, D.A. et al., “3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase of Sulfolobus solfataricus: DNA Sequence, Phylogeny, Expression inEscherichia coliof the hmgA Gene, and Purification and Kinetic Characterization of the Gene Product”, Journal of Bacteriology, Jun. 1997, vol. 179, No. 11, pp. 3632-3638.
Lang Christine
Veen Markus
Mayer & Williams PC
Meah Md. Younus
Organobalance GmbH
Wang Andrew
Wieczorek, Esq. Ann A.
LandOfFree
Method for the production of 7-dehydrocholesterol and/or the... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the production of 7-dehydrocholesterol and/or the..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the production of 7-dehydrocholesterol and/or the... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4071548