Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1982-10-19
1984-11-27
Hazel, Blondel
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
424 85, 435 68, 435811, C07C10352, A61K 4502, C12P 2100, C07G 700
Patent
active
044850387
ABSTRACT:
A process for the production and purification of human interferon derived from leukocyte of lymphoblastoid cell origin characterized by a sequence of steps involving chromatography of a solution of crude interferon on porous glass, utilizing three new classes of eluants for recovering the interferon bound to the porous glass, followed by cation exchange chromatography and finally chromatography on a hydrophobic sorbent material which process produces a final purified interferon in its natural form containing those protein components which are normally labile to low pH treatment along with those components which are pH stable. In one aspect, the improved method of purification also includes the optional molecular filtration of the crude interferon prior to initiating the purification process. In yet another embodiment, a new process for producing
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Chadha Kailash C.
Sulkowski Eugene
Hazel Blondel
Health Research (Roswell Park Division)
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