Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2004-07-30
2008-11-04
Campell, Bruce (Department: 1648)
Chemistry: molecular biology and microbiology
Vector, per se
C424S184100, C424S233100
Reexamination Certificate
active
07445930
ABSTRACT:
The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production witrh cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.
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ComplaintAventis Pharmaceuticals Products, Inc. and Aventis Pharma, S.A., Plaintiffs, v.Introgen Therapeutics, Inc., Defendant. Civil Action No. 01-451 from the U.S. District Court for the District of Delaware, Jun. 29, 2001, dismissed with prejudice Jul. 2, 2001.
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Hearing and Shenk, “F
Caston Lucetta
Cho Toohyon
Thwin Capucine
Wilson Deborah
Wu Zheng
Blumel Benjamin P
Campell Bruce
Introgen Therapeutics Inc.
Marshall & Gerstein & Borun LLP
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